By Ashok Agarwal, Sajal Gupta, Rakesh Sharma
This state of the art laboratory guide comprises 20 scientific protocols used day-by-day for the research of the infertile male, provided with effortless to appreciate, step by step technique. The protocols are prepared from regimen to complex laboratory tactics universal to scientific perform, together with computer-assisted semen research, sperm coaching for IUI through density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation try out through TUNEL strategy, between others. The technique in every one protocol follows top perform directions made clearer via professionally hand-drawn illustrations overlaying many of the very important steps and kit. The authors, hailing from the world-renowned Andrology middle at Cleveland sanatorium, have over 50 years of mixed first-hand event in handling very busy diagnostic and examine amenities in male infertility and andrology. The booklet can be an imperative source for hundreds of thousands of laboratory technologists, clinicians and reproductive pros (andrologists, embryologist, etc.) engaged within the prognosis and administration of infertile males round the world.
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Additional resources for Andrological Evaluation of Male Infertility: A Laboratory Guide
1950;20:1059–66. 41. Westgard JO, Ehrmeyer SS, Darcy TP. CLIA ﬁnal rules for quality systems. ; 2004. 42. Westgard JO, Basic QC. Practices. 2nd ed. Madison, WI: Westgard QC; 2002. 43. Knuth UA, Neuwinger J, Nieschlag E. Bias to routine semen analysis by uncontrolled changes in laboratory environment – detection by long-term sampling of monthly means for quality control. Int J Androl. 1989;12:375–83. 44. Walker RH. Pilot surveys for proﬁciency testing of semen analysis. Comparison of dry-ice vs.
8. 9. 10. Procedure Warm three tryptic soy agar (TSA) plates to room temperature for each sampling location and label with the location The negative control, biosafety hood sample, shall be collected with the hood operating normally, given adequate cycling time for the ﬁlters before use Place the plates in the sample areas and remove each lid, leave for the duration of the process being evaluated, then replace the lids Plates are incubated media side up, within a ziplock bag with a damp paper towel Incubate two plates for 48–72 h at 35 °C ± 1 °C for bacterial detection Incubate the remaining plates at room temperature for at least 5 days for mold detection When incubation is complete, count the colonies.
Testing indicators would include patterns of QC failure and instrument performance. Posttesting indicators include assessment of personnel, investigation of patient complaints, and the pregnancy rate for the IUI program. Each laboratory or the fertility program should have a QA committee that reviews the available information, researches solutions, and suggests actions to be taken to maintain and improve the quality of each test and sperm processing procedure in the laboratory. Quality management in fertility clinics has lagged behind these procedures for other pathology testing, in part due to the difﬁculty in developing appropriate quality measures for the tests performed on reproductive tract cells and tissues.
Andrological Evaluation of Male Infertility: A Laboratory Guide by Ashok Agarwal, Sajal Gupta, Rakesh Sharma