Read e-book online Advances in Macromolecular Carbohydrate Research, Volume 1 PDF

By R.J. Sturgeon

ISBN-10: 0080552277

ISBN-13: 9780080552279

ISBN-10: 1559383232

ISBN-13: 9781559383233

Lately the main major advances in carbohydrate study were made within the wisdom of the constitution and serve as of carbohydrates within the macromolecular nation. This name addresses these parts of the topic during which the authors think an important paintings is being performed.

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Extra info for Advances in Macromolecular Carbohydrate Research, Volume 1

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Subtilis, composed of a similar mixture of enzymes. Arabinoxylans Kormelink et al. [120] studied the degradation of arabinoxylan by combinations of enzymes. Arabinose releasing enzymes, be- Arabinans and Arabinan Degrading Enzymes 35 Table 12. Relative Activities of Arabinoxylan Degrading Enzymes Towards Wheat Arabinoxylan Separately and in Combinations [88]^ Enzyme AXH' Arabinofiiranosidase B ewflfo-Xylanase AXH 109 103 133 Arafur B^ endo-Xylanase 88 142 100 Notes: ^Activities of combinations are expressed in % of the sum of activities of single enzymes.

These substrates include carboxymethyl debranched arabinan, Procion Brilliant Red linear arabinan, and Remazol Brilliant Blue-or-Black-CM-linear arabinan. For practical applications we prefer the substrate dyed with Procion Red. After incubation with enzyme the unreacted substrate is precipitated in 75% ethanol and the dyed hydrolysis products are measured at 520 nm. Advanced methods to identify endo- or ejo-acting arabinanases have been introduced by the use of fast and high performance liquid chromatography (FPLC and HPLC.

58x10' 67,000 A. 0 Aral! 1 Aral! 2 Notes: "Gel filtration. ^Composed of eight subunits of 31,000 Da. ''Gel filtration results in a MW estimate of 195,000 Da. '^Composed of two subunits of 105,000 Da. ^Composed of two subunits of 46,000 Da. 'Ara I appears in Table 6. Arabinans and Arabinan Degrading Enzymes 47 earlier [137]), recent examination of the hydrolysis of arabinoxylan and isolated arabinoxylan-derived oligosaccharides by this enzyme has provided new insights into its substrate specificity.

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Advances in Macromolecular Carbohydrate Research, Volume 1 by R.J. Sturgeon


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